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Fully Automated CHO Host Cell Protein Quantitationin Monoclonal Antibody Therapeutics
Dan Schuessler, Analytical Scientist, GlaxoSmithKline, at the ForteBio Second Annual Ligand Binding Assay workshopat the AAPS National Biotechnology Conference, May 2011 The quantitation of CHO Host Cell Proteins in monoclonal antibody therapeutics is commonly performed using a laborious sandwich ELISA assay taking approximately 5.5 hours to test one 96-well assay plate. With minimal method optimization, a CHO HCP quantitation assay was developed using ForteBio Streptavidin biosensors and a Metal DAB precipitating substrate that can be completed in approximately 2 hours. Subsequently, this method was fully automated using a Hamilton liquid handling robot integrated with the Octet QK384 instrument allowing a scientist to run up to three 96 well assay plates in a single run without any user involvement after the initial setup.
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