High-Throughput Kinetic Screening of Hybridomas to Identify High-Affinity Antibodies Using Bio-Layer InterferometryLad L, et al., J Biomol Screen, 20(4):498-507, 2015
Selection and Characterization of Human PCSK9 Antibody from Phage Displayed Antibody LibraryCao Y, et al., Biochem Biophys Res Commun, 7;463(4):712-8, 2015
Exposure of Epitope Residues on the Outer Face of the Chikungunya Virus Envelope Trimer Determines Antibody Neutralizing EfficacyFong R, et al., J Virol, 88(24):14364-79, 2014
REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast CellsRhiel L, et al., PLoS One, 9(12):e114887, 2014
High-content Analysis of Antibody Phage-display Library Selection Outputs Identifies Tumor Selective Macropinocytosis-dependent Rapidly Internalizing AntibodiesHa K, et al., Mol Cell Proteomics, 13(12):3320-31, 2014
Monoclonal Antibody Targeting of Fibroblast Growth Factor Receptor 1c Ameliorates Obesity and Glucose Intolerance via Central MechanismsLelliott C, et al., PLoS One, 9(11):e112109, 2014
A Novel Rabbit Monoclonal Antibody Platform To Dissect the Diverse Repertoire of Antibody Epitopes for HIV-1 Env Immunogen DesignChen Y, et al., J Virol, 87(18), 10232-43, 2013
Expression of High-Affinity Human Antibody Fragments in BacteriaR Rouet, et al., Nature Protocols, 7(2), 364-373, 2012
Identification of Anti-Alpha Toxin Monoclonal Antibodies that Reduce the Severity of Staphylococcus Aureus Dermonecrosis and Exhibit a Correlation Between Affinity and Potency C Tkaczyka, et.al., Clin Vaccine Immunol, 19(3), 377-385, 2012
Development and Evaluation of Monoclonal Antibodies against Phosphatidylethanolamine Binding Protein 1 in Pancreatic Cancer PatientsWang, X., et al., J Immunological Methods, 362, 151-160, 2010
Cell-Free Translational Systems for Rapid Species Cross-Reactivity Screening
Kinetic Characterization of Protein-Protein Interactions in Purified and Bacterial Lysates
Ryan Case, Ph.D., Amgen, 2011 This talk presents the label-free, real-time monitoring capabilities of the Octet RED system for kinetic characterization of a variety of protein-protein interactions spanning low, medium and high affinity to identify lead candidates in biotherapeutics drug discovery. Kinetic information often reveals details of interactions missed in a purely affinity-based ELISA screens that are important in ranking clones. The use of the Octet RED system for off-rate screening of proteins in bacterial lysates will also be discussed.
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