For Label-Free Immobilization and Subsequent Kinetic Characterization of Human IgG or other Human Fc-containing Proteins
Anti-Human IgG Fc Capture (AHC) biosensors enable kinetic characterization of macromolecular interactions between human Fc-containing proteins and target analytes. Immobilization of human Fc-containing proteins is achieved through a factory immobilized anti-human Fc-specific antibody whose high-affinity for the human Fc domain provides the stable baseline required for demanding kinetics applications. Cost-effective regeneration of the biosensors and the ability to directly immobilize human Fc-containing proteins from crude matrices make the AHC biosensor extremely useful in high-throughput applications.
- Capture-based immobilization of human Fc-containing proteins
- Regenerable and cost-effective format
- Compatible with crude samples and complex media
Figure 1: Kinetic characterization of the interaction between hIgG and an analyte using AHC biosensors within a five-step assay. Step 1: baseline, Step 2: loading of hIgG, Step 3: baseline, Step 4: association kinetics, Step 5: dissociation kinetics. Biosensors A (blue, buffer matrix) and B (orange, CD-CHO containing FBS) delivered similar values for loading, association and dissociation steps. Reference biosensors C (gold, buffer matrix) and D (maroon, CD-CHO containing FBS) delivered low background values, indicating that binding of hIgG and the analyte are protein-specific interactions.
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