For Quantitation and Kinetic Characterization of Human Fab, F(ab′)2 and IgG
The detection and characterization of human antibodies is vital to drug discovery and biotherapeutics development efforts. The Anti-Human Fab-CH1 Biosensor enables quick and easy detection and quantitation of human Fab, F(ab′)2 and IgG or their capture for affinity measurements with other analytes for kinetic characterization. The biosensor has no recognition towards free light chains or cross-reactivity with murine and bovine IgG, making it particularly suitable for quantitation of crude samples taken directly from cell expression.
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- Rapid quantitation of all subclasses of human Fab, F(ab′)2 and IgG
- Easy capture of human Fab, F(ab′)2 and IgG for kinetic analysis with antigen
- No recognition towards human kappa or lambda light chains
- No cross-reactivity towards bovine or mouse IgG
Figure 1: Detection of a F(ab′)2 standard using Anti-Human Fab-CH1 biosensors on an Octet RED384 system (1000 rpm, 2 minutes) for a standard dynamic range. A) F(ab′)2 dose. B) The resulting calibration curve. Sample diluent was used as a matrix for all samples.
Figure 2: Kinetic analysis of the interaction between ligand HIgG F(ab′)2 (110 kDa) and an analyte F(ab′)2 fragment, Goat anti-HIgG F(ab′)2 specific (110 kDa). 1X Kinetics Buffer was used as the matrix throughout and the assay temperature was 30°C. Data were processed and the curve fit using a 1:1 binding model. Kinetic results for the interaction between ligand HIgG F(ab′)2 (110 kDa) and an analyte using Anti-Human Fab-CH1 biosensors are reported in the table.
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